Captain Christopher Pike
Played by: Jeffrey Hunter

Yeoman J. Mia Colt
Played by: Laurel Goodwin

Lieutenant Jose Tyler
Played by: Peter Duryea

Transporter Chief Nils Pitcairn
Played by: Clegg Hoyt

Dr. Philipp Boyce
Played by: John Hoyt

Mr. Spock
Played by: Leonard Nimoy

The Keeper
Played by: Meg Wyllie

First Officer Una Chin-Riley (Number One)
Played by: Majel Barrett

Vina
Played by: Susan Oliver

Geologist Edward Fisher
Played by: Edward (Ed) Madden

C.P.O. Garison
Played by: Adam Roarke

The Keeper [Voice]
Played by: Malachi Throne

Dr. Theodore Haskins
Played by: Jon Lormer

[Unnamed Anthropoid Ape 01]
Played by: Janos Prohaska

[Unnamed Human Starfleet Officer 01]
Played by: Robert Phillips

[Unnamed Human Survivor 02]
Played by: Leonard Mudie

[Unnamed Human Survivor 03]
Played by: Anthony Jochim

[Unnamed Human Survivor 04]
Played by: Joe Evans

[Unnamed Human Survivor 05]
Played by: William McCarter

[Unnamed Human Survivor 06]
Played by: William H. O'Brien

[Unnamed Human Survivor 07]
Played by: Sam Bagley

[Unnamed Human Trader 01]
Played by: Joseph Mell

[Unnamed Humanoid Bird 01]
Played by: Janos Prohaska

[Unnamed Kalar 01]
Played by: Michael Dugan

[Unnamed Talosian 01]
Played by: Georgia Schmidt

[Unnamed Talosian 01] (Voice)
Played by: Robert (Bob) C. Johnson

[Unnamed Talosian 02]
Played by: Serena Sande

[Unnamed Talosian 03]
Played by: Felix Silla

Crew Member [Unnamed USS Enterprise Operations Crewman 02]
Played by: John Burnside

Crew Member [Unnamed USS Enterprise Operations Crewwoman 03]
Played by: Carol Daniels