Nurse Christine Chapel
Played by: Majel Barrett

Lieutenant Hikaru Sulu
Played by: George Takei

Captain James Tiberius Kirk
Played by: William Shatner

Yeoman Janice Rand
Played by: Grace Lee Whitney

Dr. Leonard 'Bones' McCoy
Played by: DeForest Kelley

Engineer Montgomery 'Scotty' Scott
Played by: James Doohan

Communications Officer Nyota Uhura
Played by: Nichelle Nichols

Mr. Spock
Played by: Leonard Nimoy

Balok
Played by: Clint Howard

Balok (First Voice)
Played by: Ted Cassidy

Lieutenant Dave Bailey
Played by: Anthony Call

Balok (Final Voice)
Played by: Walker Edmiston

Lieutenant Bill Hadley
Played by: William (Bill) Blackburn

Crew Member Clifford Brent
Played by: Frank da Vinci

Crew Member Harrison
Played by: Ron Veto

Lieutenant Ryan Leslie
Played by: Eddie Paskey

Crew Member [Unnamed Human Crew Member 06]
Played by: Sean Morgan

Yeoman [Unnamed Human Crew Member 07]
Played by: Jeannie Malone

Lieutenant [Unnamed Human Crew Member 08]
Played by: Frieda Rentie