Captain James Tiberius Kirk
Played by: William Shatner

Yeoman Janice Rand
Played by: Grace Lee Whitney

Dr. Leonard 'Bones' McCoy
Played by: DeForest Kelley

Communications Officer Nyota Uhura
Played by: Nichelle Nichols

Mr. Spock
Played by: Leonard Nimoy

Charlie Evans
Played by: Robert Walker Jr.

Lieutenant Bill Hadley
Played by: William (Bill) Blackburn

Captain Ramart
Played by: Charles Stewart

Sam Fuller
Played by: Bob Herron

Yeoman Tina Lawton
Played by: Pat McNulty

Lieutenant Tom Nellis
Played by: Dallas Mitchell

Crew Member Vinci
Played by: Frank da Vinci

Crew Member Wilson
Played by: Garland Thompson

Crew Member [Unnamed Human Crew Member 12]
Played by: John Bellah

Navigator [Unnamed Human Crew Member 13]
Played by: Don Eitner

Helmsman [Unnamed Human Crew Member 15]
Played by: John Lindesmith

[Unnamed Thasian 01]
Played by: Abraham Sofaer

Operations Divison Lieutenant [Unnamed USS Enterprise Lieutenant 05]
Played by: Robert Metz